鑽у厜鍘熶綅鏉備氦绠€浠婞/h1>

1銆佸畾涔堻/p>

鑽у厜鍘熶綅鏉備氦锛坒luorescent in situ hybridization锛孎ISH锛 鏄竴绉嶇粏鑳為仐浼犲鎶€鏈紝鍙互鐢ㄦ潵瀵规牳閰歌繘琛屾娴嬪拰瀹氫綅銆傝崸鍏夋爣璁扮殑鏍搁吀鎺㈤拡鍙拰鍏锋湁楂樺害鐩镐技鎬х殑鏍搁吀鏉備氦锛屽彲鐢ㄤ簬鏌撹壊浣撲笂鍩哄洜鐨勫畾浣嶏紝鎴栧湪鍒嗗瓙鐢熸€佸涓敤鏉ユ爣璁颁笉鍚屽垎绫荤粏鑿屾垨鍙よ弻涓殑鏍哥硸浣揜NA銆?nbsp;

    

2銆佸師鐞咟/p>

FISH(fluorescence in situ hybridization)鎶€鏈槸涓€绉嶉噸瑕佺殑闈炴斁灏勬€у師浣嶆潅浜ゆ妧鏈€傚畠鐨勫熀鏈師鐞嗘槸:濡傛灉琚娴嬬殑鏌撹壊浣撴垨DNA绾ょ淮鍒囩墖涓婄殑闈禗NA涓庢墍鐢ㄧ殑鏍搁吀鎺㈤拡鏄悓婧愪簰琛ョ殑锛屼簩鑰呯粡鍙樻€?閫€鐏?澶嶆€э紝鍗冲彲褰㈡垚闈禗NA涓庢牳閰告帰閽堢殑鏉備氦浣撱€傚皢鏍搁吀鎺㈤拡鐨勬煇涓€绉嶆牳鑻烽吀鏍囪涓婃姤鍛婂垎瀛愬鐢熺墿绱犮€佸湴楂樿緵锛屽彲鍒╃敤璇ユ姤鍛婂垎瀛愪笌鑽у厜绱犳爣璁扮殑鐗瑰紓浜插拰绱犱箣闂寸殑鍏嶇柅鍖栧鍙嶅簲锛岀粡鑽у厜妫€娴嬩綋绯诲湪闀滀笅瀵瑰緟娴婦NA杩涜瀹氭€с€佸畾閲忔垨鐩稿瀹氫綅鍒嗘瀽銆侟/p>

3銆佹剰涔堻/p>

鍘熶綅鏉備氦鐨勬帰閽堟寜鏍囪鍒嗗瓙绫诲瀷鍒嗕负鏀惧皠鎬ф爣璁板拰闈炴斁灏勬€ф爣璁般€傜敤鍚屼綅绱犳爣璁扮殑鏀惧皠鎬ф帰閽堜紭鍔垮湪浜庡鍒跺鏍峰搧鐨勮姹備笉楂橈紝鍙互閫氳繃寤堕暱鏇濆厜鏃堕棿鍔犲己淇″彿寮哄害锛屾晠杈冪伒鏁忋€傜己鐐规槸鎺㈤拡涓嶇ǔ瀹氥€佽嚜鏄惧奖鏃堕棿闀裤€佹斁灏勭嚎鐨勬暎灏勪娇寰楃┖闂村垎杈ㄧ巼涓嶉珮銆佸強鍚屼綅绱犳搷浣滆緝绻佺悙绛夈€傞噰鐢ㄨ崸鍏夋爣璁扮郴缁熷垯鍙厠鏈嶈繖浜涗笉瓒筹紝杩欏氨鏄疐ISH鎶€鏈€侳ISH鎶€鏈綔涓洪潪鏀惧皠鎬ф娴嬩綋绯伙紝鏈変互涓嬬壒鐐广€侟/p>

4銆佷紭鐐更/p>

1銆佽崸鍏夎瘯鍓傚拰鎺㈤拡缁忔祹銆佸畨鍏紱
2銆佹帰閽堢ǔ瀹氾紝涓€娆℃爣璁板悗鍙湪涓ゅ勾鍐呬娇鐢紱
3銆佸疄楠屽懆鏈熺煭銆佽兘杩呴€熷緱鍒扮粨鏋溿€佺壒寮傛€уソ銆佸畾浣嶅噯纭紱
4銆丗ISH鍙畾浣嶉暱搴﹀湪1kb鐨凞NA搴忓垪锛屽叾鐏垫晱搴︿笌鏀惧皠鎬ф帰閽堢浉褰擄紱
5銆佸鑹睩ISH閫氳繃鍦ㄥ悓涓€涓牳涓樉绀轰笉鍚岀殑棰滆壊鍙悓鏃舵娴嬪绉嶅簭鍒楋紱
6銆佹棦鍙互鍦ㄧ幓鐗囦笂鏄剧ず涓湡鏌撹壊浣撴暟閲忔垨缁撴瀯鐨勫彉鍖栵紝涔熷彲浠ュ湪鎮恫涓樉绀洪棿鏈熸煋鑹蹭綋DNA鐨勭粨鏋勩€?nbsp;

5銆佺己鐐更/p>

涓嶈兘杈惧埌100%鏉備氦锛岀壒鍒槸鍦ㄥ簲鐢ㄨ緝鐭殑cDNA鎺㈤拡鏃舵晥鐜囨槑鏄句笅闄嶃€侟/p>