绌洪棿杞綍缁勬祴搴廃/strong>
鍩哄洜琛ㄨ揪鍏锋湁鏃堕棿鍜岀┖闂寸殑鐗瑰紓鎬с€傞€氳繃瀵逛笉鍚屾椂闂寸偣鍜岄儴浣嶇殑鏍锋湰鍙栨潗锛屼娇鐢ㄥ崟缁嗚優杞綍缁勬祴搴忔妧鏈兘澶熻В鏋愭椂闂寸淮搴︿笂缁嗚優绫诲瀷鍜屽熀鍥犺〃杈剧殑鍙樺寲杩囩▼銆傜劧鑰屽崟缁嗚優娴嬪簭瀹為獙鐨勫墠鎻愭槸缁勭粐蹇呴』閫氳繃鏈烘鍒嗙鎴栭叾瑙ｆ秷鍖栨垚鍗曠粏鑳炴偓娑诧紝姝よ繃绋嬩笉鍙伩鍏嶅湴涓㈠け浜嗙粍缁囦腑缁嗚優鎵€澶勭殑鍘熷浣嶇疆淇℃伅锛屼篃瀵艰嚧浜嗙粏鑳為棿鐨勯€氳缃戠粶琚墦鐮达紝杩欎娇鎴戜滑闅句互鑾峰緱缁勭粐涓笉鍚屽尯鍩熺殑缁嗚優鏋勬垚鍜屽熀鍥犺〃杈剧姸鎬侊紝浠ュ強涓嶅悓鍔熻兘鍖轰箣闂寸殑鍩哄洜宸紓琛ㄨ揪绛変俊鎭€傜幇鏈夌殑鍘熶綅琛ㄨ揪鍥捐氨涓昏鏄€氳繃鎶ュ憡鍩哄洜鎴栧師浣嶆潅浜ょ瓑鎶€鏈潵瀹炵幇锛屼絾鏄繖浜涙柟娉曞疄鐜版瘮杈冨洶闅撅紝骞朵笖閫氶噺浣庯紝闄愬埗浜嗗鏍锋湰銆侀珮鏃舵晥鐨勫簲鐢ㄣ€傝€岀┖闂磋浆褰曠粍鎶€鏈垯鍙互楂樻晥鍦版娴嬬粍缁囦腑绌洪棿鍘熷浣嶇疆涓婄殑鍩哄洜琛ㄨ揪妯″紡銆侟/span>
鍥?.绌洪棿杞綍缁勬祴搴忔妧鏈摤鍕冨彂灞旤/span>
绌洪棿杞綍缁勶紙Spatial Transcriptomics锛夊氨鏄皢鍩哄洜鐨勮〃杈炬儏鍐典笌鍏虫敞鐨勭粍缁囧垏鐗囩殑鍏嶇柅鍖栧鏌撹壊鍥惧儚杩涜鏁村悎锛屼粠鑰屽皢缁勭粐鍐呬笉鍚岀粏鑳炵殑鍩哄洜琛ㄨ揪淇℃伅瀹氫綅鍒扮粍缁囩殑鍘熷绌洪棿浣嶇疆涓婂幓锛岃繘鑰岀洿鎺ヨ娴嬬粍缁囦腑涓嶅悓閮ㄤ綅鍔熻兘鍖哄熀鍥犺〃杈剧殑宸紓銆傜┖闂磋浆褰曠粍鍒╃敤浜嗗父瑙勭殑鍘熶綅鎶€鏈拰缁勫鎶€鏈袱鏂归潰鐨勪紭鍔匡紝缁撳悎浜嗚姱鐗囦笌楂橀€氶噺娴嬪簭鐨勬牳蹇冩蹇点€侟/span>
1.鎶€鏈紭鍔军/span>
鈶犮€€骞冲彴绋冲畾锛氫笉闇€瑕佸崟缁嗚優瑙ｇ锛岀粍缁囧垏鐗囧嵆鍙紱
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2.鎶€鏈師鐞咟/span>
灏嗗喎鍐荤粍缁囧垏鐗囨斁缃湪10X Genomics Visium鑺墖鐨勬崟鑾峰尯鍩熷唴锛孅/span>杩涜HE鏌撹壊鍜屾垚鍍忓悗锛孅/span>瀵圭粍缁囧垏鐗囪繘琛岄€忓寲澶勭悊锛孅/span>缁嗚優鍐呯殑mRNA鍥犺€孅/span>閲婃斁锛孅/span>浠庤€岃鑺墖涓婂甫鏈堻/span>oligo-dT鐨勬帰閽堟崟鑾饵/span>銆侟/span>骞朵笖锛孅/span>姣忎釜鎺㈤拡閮藉甫鏈夌壒寮傜殑浣嶇疆淇℃伅锛圏/span>Spatial Barcode锛夛紝鐒跺悗浠?/span>mRNA涓烘ā鐗堣繘琛宑DNA鍚堟垚锛孅/span>鏋勫缓鏂囧簱鍚庡啀娴嬪簭銆傛祴搴廃/span>鑾峰緱鍩哄洜琛ㄨ揪淇℃伅鐨勫悓鏃迭/span>锛孅/span>姣忎竴鏉℃祴搴廃/span>reads鍥犲甫鏈塖patial Barcode锛岃繘琛屽姣斿嵆鍙?/span>鑾峰緱鍩哄洜琛ㄨ揪鐨勪綅缃俊鎭€侟/span>
鍥?.绌洪棿杞綍缁勬暣浣撳疄楠屾祦绋婞/span>
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鍥?.绌洪棿杞綍缁勮姱鐗囨瀯鎴忺/span>
3.鑱斿悎鍗曠粏鑳炴祴搴忚В鏋愮粏鑳炵被鍨嬬殑绌洪棿浣嶇疆淇℃伅
绌洪棿杞綍缁勬祴搴忓彲浠ヨ幏寰椾笉鍚屽熀鍥犲湪缁勭粐鍒囩墖涓婄殑绌洪棿浣嶇疆淇℃伅锛孅/span>浣嗕笉鑳借幏寰楄缁嗙殑缁嗚優绫荤兢淇℃伅锛堝洜涓裹/span>绌洪棿杞綍缁勪笉鏄崟缁嗚優鍒嗚鲸鐜嘃/span>锛屽畠浠?/span>spot涓哄崟浣嶈繘琛屽垎杈紝鍙兘绮楃暐鍦?/span>鍒嗘瀽鍒囩墖涓婁笉鍚屼綅缃殑缁嗚優绫诲瀷锛堻/span>銆傚洜姝｜/span>锛孅/span>闇€瑕佸€熷姪鍗曠粏鑳炴祴搴忔暟鎹潵鍒嗘瀽缁嗚優绫诲瀷锛孅/span>鐒跺悗閫氳繃鐢熺墿淇℃伅瀛︾殑鍒嗘瀽鏂规硶灏嗗崟缁嗚優绫荤兢鏄犲皠鍒扮┖闂磋浆褰曠粍鏁版嵁涓娿€侟/span>
閫夋嫨宸叉湁鐨勫搴旂墿绉嶃€佺粍缁囩殑缁嗚優绫诲瀷娉ㄩ噴鏁版嵁浣滀负鍙傝€冨簱銆傚熀浜庡崟缁嗚優娴嬪簭寰楀埌鐨勪笉鍚岀被缇ょ殑Marker Gene锛岄€夋嫨鍙傝€冩暟鎹簱涓笉鍚岀粏鑳炵被鍨嬮棿楂樺害鍙樺寲鐨勫熀鍥狅紝鐒跺悗璁＄畻棰勬祴鐨凕/span>spot涓庡弬鑰冩暟鎹簱鐨勭浉鍏虫€э紝閫氳繃涓嶆柇鍓旈櫎鐩稿叧鎬ф渶宸殑绫诲瀷浠ュ惊鐜绠楃浉鍏虫€э紝鏈€缁堝緱鍒伴娴媠pot鐨勭粏鑳炵被鍨嬫敞閲夵/span>銆侟/span>
鍥?.MIA(Multimodal intersection analysis)